primary human pulmonary artery endothelial cells (hpae)  (Lonza)

Journal: Cell reports

Article Title: FAK regulates tension transmission to the nucleus and endothelial transcriptome independent of kinase activity

doi: 10.1016/j.celrep.2024.114297

Figure Lengend Snippet:

Article Snippet: Primary Human Pulmonary Artery Endothelial Cells (HPAE) , Lonza Bioscience , Cat# CC-2530.

Techniques: Blocking Assay, Control, Recombinant, Activation Assay, SYBR Green Assay, Reverse Transcription, Plasmid Preparation, Transfection, Contraction Assay, Extraction, Activity Assay, Methylation, Software

Journal: iScience

Article Title: Endothelial cell senescence exacerbates pulmonary hypertension by inducing juxtacrine Notch signaling in smooth muscle cells

doi: 10.1016/j.isci.2023.106662

Figure Lengend Snippet: Senescent ECs enhance SMCs proliferation and migration through direct cell-cell contact (A) Real-time qPCR analysis for CDKIs and SASP factors in pulmonary artery ECs (PAECs) transfected with either GFP (control cells) or TRF2DN (premature senescent cells) (n = 5–8 each). (B) Schemes for co-culture experiments of PAECs and pulmonary artery SMCs (PASMCs). (C) Immunocytochemistry for Ki-67 in PASMCs directly (n = 7 each) or indirectly (n = 5 each) co-cultured with control or premature senescent PAECs. (D) Migration capacity was assessed by a modified Boyden chamber assay in PASMCs directly or indirectly co-cultured with control or premature senescent PAECs (n = 3 each). (E) Real-time qPCR analysis for Notch ligands in PAECs transfected with either GFP (control cells) or TRF2DN (premature senescent cells) (n= 6–8 each). (F) Real-time qPCR for Notch target genes in PASMCs directly or indirectly co-cultured with control or premature senescent PAECs (n = 4–5 each). Data are presented as mean ± SEM. Two-tailed Student’s t test was used for the analysis of the differences between two groups. Two-way ANOVA with Tukey’s post hoc test was used for the analysis of the differences between groups more than three. ∗ p <0.05, ∗∗ p <0.01, ∗∗∗ p <0.001, ∗∗∗∗ p <0.0001, and ns; not significant. See also Figures S5–S7 .

Article Snippet: Human pulmonary arterial endothelial cells (PAECs) and smooth muscle cells (PASMCs) were purchased from PromoCell.

Techniques: Migration, Transfection, Co-Culture Assay, Immunocytochemistry, Cell Culture, Modification, Boyden Chamber Assay, Two Tailed Test

Journal: iScience

Article Title: Endothelial cell senescence exacerbates pulmonary hypertension by inducing juxtacrine Notch signaling in smooth muscle cells

doi: 10.1016/j.isci.2023.106662

Figure Lengend Snippet: Notch signaling plays a crucial role in the pathological interaction between senescent ECs and SMCs (A) Immunocytochemistry for Ki-67 in PASMCs directly co-cultured with control (GFP) or premature senescent (TRF2DN) PAECs with or without IL-1a gene silencing (n = 3 each). (B) Migration capacity was assessed by a modified Boyden chamber assay in PASMCs directly co-cultured with control (GFP) or premature senescent (TRF2DN) PAECs with or without IL-1a gene silencing (n = 9–11 each). (C) Immunocytochemistry for Ki-67 in PASMCs directly co-cultured with control (GFP) or premature senescent (TRF2DN) PAECs (n = 3–4 each). PASMCs were infected with lentiviruses delivering either empty or Notch3 shRNA. (D) Migration capacity was assessed by a modified Boyden chamber assay in PASMC co-cultured with control (GFP) or premature senescent (TRF2DN) PAECs (n = 10–12 each). PASMCs were infected with lentiviruses delivering either empty or Notch3 shRNA. Data are presented as mean ± SEM. One-way ANOVA was used for the analysis of the differences between three groups. Two-way ANOVA with Tukey’s post hoc test was used for the analysis of the differences between groups more than three. ∗ p <0.05, ∗∗ p <0.01, ∗∗∗ p <0.001, and ∗∗∗∗ p <0.0001. See also Figure S8 .

Article Snippet: Human pulmonary arterial endothelial cells (PAECs) and smooth muscle cells (PASMCs) were purchased from PromoCell.

Techniques: Immunocytochemistry, Cell Culture, Migration, Modification, Boyden Chamber Assay, Infection, shRNA